Stones, 28, was recalled to the starting XI against Germany by Gareth Southgate after missing the Italy match due to suspension. However, his return lasted 37 minutes before the centre-back was withdrawn.
Stones started a counter-attack for Gareth Southgate’s side by making an interception on the edge of his own penalty box and then looked to get up the pitch but quickly pulled up clutching the back of his leg.
Walker, who has just returned from a muscle injury, started England’s 1-0 defeat to Italy on Friday.
Both Stones and Walker have seen the start to their season disrupted by injury.
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